Other studies validating the ActiGraph have found vastly different cut-points for SB (range 50–250 counts/min) and MVPA (191–2,691 counts/min) in adults, depending on the population and type of validation setting (55, 56). The cut-point method has several limitations; it cannot differentiate standing from sitting/lying, but standing is considered LPA because it elicits different physiologic responses and has different long- term health consequences than sitting/lying (57, 58).