AbstractThe aim of this study was t

Abstract

The aim of this study was to observe the effects of re-combinant human endostatin on the proliferation and apoptosis of mouse gastric cancer cells, and explore some possible mechanisms of recom-binant human endostatin inhibition of cancer. A murine gastric cancer xenograft model was established. A total of 20 mice were divided into two groups (control and experimental groups). The expression of c-Myc and basic fibroblast growth factor (bFGF) was determined by reverse transcription-polymerase chain reaction, Western blotting, and immu-nohistochemical staining methods. Tumor volume was measured and a growth curve was calculated. The tumor diameter in the experimental group was significantly smaller than that in the control group after treat-ment with endostatin for 21 days. The expression levels of c-Myc and bFGF in the experimental group were significantly lower than those of the control group (P < 0.05). There was a positive correlation between the expression of c-Myc and bFGF in the experimental group. Microvessel density was significantly inhibited in the experimental group (P < 0.05). These results demonstrated that recombinant human endostatin could in-hibit tumor metastasis by inhibition of the expression of c-Myc and bFGF in gastric cancer tissue as well as by inhibition of angiogenesis.

結果 (中文) 1:

摘要本研究旨在观察重新联合人内皮抑素对扩散的影响和对小鼠胃癌细胞凋亡，探讨侏儒症人内皮抑素抑制癌症的一些可能的机制。建立了小鼠胃癌裸鼠移植瘤模型。共 20 老鼠分为两组 (对照组和实验组)。用逆转录-聚合酶链反应、 免疫印迹，和免疫 nohistochemical 染色方法测定了 c-Myc 和基本的成纤维细胞生长因子 (bFGF) 的表达。测量肿瘤体积和生长曲线计算。实验组肿瘤直径后 21 天内皮抑素治疗发言明显小于对照组。C-Myc 和实验组 bFGF 的表达水平均低于对照组 (P < 0.05)。有了 c-Myc 的表达和 bFGF 在实验组之间的正相关。微血管密度明显抑制实验组 (P < 0.05)。这些结果表明，重组人内皮抑素能在胞浆肿瘤转移，c-Myc 和 bFGF 在胃癌组织中的表达的抑制作用，抑制血管生成。

結果 (中文) 3:

摘要

本研究的目的是观察重组人内皮抑素对小鼠胃癌细胞增殖凋亡的影响，探讨重组人内皮抑素抑制某些癌症的可能机制。建立小鼠胃癌移植瘤模型。将20只小鼠分为对照组和实验组。c-myc和碱性成纤维细胞生长因子（bFGF）的表达，采用逆转录聚合酶链反应测定，免疫印迹和免疫组化染色方法。测量肿瘤体积并计算生长曲线。实验组肿瘤直径明显小于对照组，天内治疗后，内皮细胞瘤直径为21天。实验组中c-myc和bFGF的表达水平显著低于对照组（P＜0.05）。实验组中c-myc和bFGF的表达呈正相关。实验组微血管密度明显抑制（0.05）。这些结果表明，重组人血管内皮抑素能抑制肿瘤转移的胃癌组织中c-myc和bFGF表达的抑制作用以及对血管生成的抑制作用。