Finally, the TASCC approach is not limited to slug additions only. It may be applied to constant-rate BTCs in the same fashion as slug BTC data, so long as grab samples were/are collected on the rising and falling limbs to and from plateau conditions. Also, TASCC can be applied with other biologically active tracers (e.g., P, NH4, urea, acetate, etc), which could be particularly useful for quantifying P spiraling curves, given the use of P radioisotopes (32P, 33P) is impractical due to health concerns.