All determinations were performed in duplicate. Platelet function tests were carried out to quantify platelet activation levels without any stimulus and to estimate their tendency to become activated by a physiologically relevant stimulus to test platelet reactivity. For measurement
of basal platelet activation, phosphate-buffered saline (PBS) was applied instead of agonists. For assessment of platelet reactivity, platelet agonist adenosine diphosphate (ADP,
Sigma Aldrich, Vienna, Austria) was incubated with platelets at different concentrations (final concentrations: 2.5, 5 and 50 μM) for 10 min.