Procedure for streaking plates for

Procedure for streaking plates for primary isolation

1. Sterilize the inoculating loop in the incinerator for 5 to 10s. Allow the loop to cool thoroughly before streaaking medium. A sterile plastic loop can be used in place of the sterile inoculating loop.

2. Pass the cooled loop back and forth through the inoculum in the first quadrant several times.

3. Do not flame the loop between the quadrants unless necessary.

4. Turn the plate a quarter turn, and pass the loop through the edge of the first quadrant approximately four times while streaking into the second quadrant. Continue streaking in the second quadrant without going back to first quadrant, approximately four times.

5. Turn the plate another quadrant turn, and pass the loop through the edge of the second quadrant approximately four times while streaking into the third quadrant.
Continue streaking in the third quadrant without going back into the second quadrant, approximately four times.

6. Flame the loop between plates to prevent carrying over a possible contaminant from the previous plate.

7. Continue streaking the rest of the culture media in the same manner

8. Inoculate suitable nutrient broth with 1 to 2 loopfuls of specimen or swab.

結果 (繁體中文) 1:

裸奔的原代分離板的程式1.消毒接種迴圈中的焚化爐，進行 5 到 10 秒。允許迴圈涼透後才 streaaking 介質。一個無菌的塑膠環可代替無菌接種環。2.通過冷卻的迴圈通過接種來回在第一象限幾次。3.做不火焰之間象限的回路，除非必要。4.轉盤子四分之一轉，並通過第一象限的邊緣遍歷大約四倍同時進入第二象限裸奔。繼續回到第一象限，在第二象限無去裸奔大約四倍。5.打開另一個象限轉，板和通過第二象限的邊緣遍歷大約四倍同時裸奔入第三象限。繼續裸奔在第三象限不用回第二象限，約四倍。6.火焰板，可防止攜帶超過可能的污染物從以前板之間的回路。7.繼續裸奔其他的文化媒體以同樣的方式8.接種適宜營養肉湯中用 1 到 2 loopfuls 的標本或拭子。

結果 (繁體中文) 2:

程序裸奔板的主要隔離1。消毒在焚燒爐的接種環為5至10秒。允許該環streaaking介質之前徹底冷卻。無菌塑料環可以代替無菌接種環的使用。2。通過將冷卻環來回通過在第一象限數次接種物。3。不熄火的象限之間的循環，除非必要的。4。轉動板四分之一圈，並通過第一象限的邊緣通過循環大約四倍，同時劃線到第二象限。繼續裸奔在第二象限，而無需返回到第一象限，約四倍。5。轉動板另一象限轉，並通過第二象限的邊緣通過循環大約四倍，同時劃線到第三象限。繼續條紋在第三象限，而不必返回到第二象限，大約四倍。6。火焰板之間的循環，以防止攜帶從上一個板的可能的污染物。7。繼續拖尾中相同的方式的培養基的其餘部分8。接種合適的營養肉湯與樣品或拭子的1至2個環量。