Audiometric HTLs in call center operators were compared to age-related reference data from highly screened and unscreened populations according to ISO 7029:2017[15] and ISO 1999:2013.[16] Differences in HTLs between participants’ left and right ears as well as between ears exposed and nonexposed to noise from headsets were also explored.The prevalence of normal audiograms, high- and speech-frequency hearing loss were analyzed among call center operators. Normal hearing was defined as having mean HTL between 0.25 and 8 kHz lower than or equal to 20-dB HL. In contrast, speech- and high-frequency hearing loss were defined as pure-tone mean of >20 dB HL at 0.5, 1, 2, and 4 kHz, and 3, 4, and 6 kHz, respectively. Percentages of ears with HTLs exceeding 20 dB HL at any of high frequencies (3–6 kHz) and at speech frequencies (0.5, 1, 2, and 4 kHz) were also calculated.