摘 要 目的 探讨肺炎支原体（MP）咽拭子快速液体培养法、咽拭子聚合酶

摘 要


目的 探讨肺炎支原体（MP）咽拭子快速液体培养法、咽拭子聚合酶链反应（PCR）法和血清MP被动凝集法（MP-Ab）等方法在儿童MP感染诊断治疗过程中的敏感性方法 采用3MP检测法对362例临床拟诊呼吸道（非细菌性）感染的患儿的咽拭子和血清标本进行配对研究，每患儿取咽拭子做快速培养和PCR，同时取血清做MP-Ab检测，对3种方法的检测结果与临床诊断治疗病例进行回顾性分析。结果 回顾临床病例诊断MP感染患儿152例。MP快速培养法检出阳性78例，阳性率为51.3%，病程为（4.5±2.6）天；PCR法检测出阳性103例，阳性率为67.8%，病程为（6.2±3.5）天；MP-Ab法检测出阳性127例，阳性率83.5%，病程（8.1±4.5）天。结论 3种MP检测法的敏感性与病程有相关性，临床医生应根据患儿病程选取检测方法，以提高阳性检出率和敏感性。

关键词 肺炎支原体，快速培养法，咽拭子聚合酶链反应，血清抗体，配对研究

結果 (中文) 3:

AbstractObjective to investigate mycoplasma pneumonia (MP) pharyngeal swab rapid liquid culture method, pharyngeal swab polymerase chain reaction (PCR) method and serum MP Passive Agglutination Method (AB) method in children with MP infection diagnosis treatment sensitivity method using 3MP detection method for 362 cases of clinically suspected call respiratory tract (non bacterial infection in children with throat swabs and serum samples were studied by matched, every children take throat swab rapid culture and PCR, and serum AB detection, the detection results of the three methods and clinical diagnosis cases were retrospective analysis. Results 152 cases of children with MP infection were reviewed retrospectively. MP rapid culture assay for detection of 78 cases were positive, the positive rate was 51.3%, duration (4.5 + 2.6 days; PCR method for the detection of the 103 cases were positive, the positive rate was 67.8%, duration (6.2 + 3.5 days; AB was detected out of 127 cases were positive, positive rate was 83.5%, duration of 8.1 + 4.5 days. Conclusion the sensitivity of the 3 MP detection methods is related to the course of the disease, clinicians should choose the detection method according to the course of disease, in order to improve the positive detection rate and sensitivity.Key words Mycoplasma pneumoniae, rapid culture method, throat swab polymerase chain reaction, serum antibody, paired study