Vanilla planifolia multiplication t

Vanilla planifolia multiplication through tissue culture was worked out with new leads in elucidation of synergistic activity of zeatin, synthetic cytokinin thidiazuron (TDZ) and coconut milk (CM) as well as N6-benzyladenine(BA) . Multiple shoots were developed from axillary bud explants using Murashige and Skoog (MS) medium supplemented with zeatin, BA+zeatin , TDZ and TDZ+ coconut milk (CM). The nature of explant and the method of explant inoculation onto the medium influence not only multiple shoot production, but also bulbous shoot buds (BS) formation. Zeatin supported the growth of mostly single shoots, whereas formation of BS was induced in zeatin and BA combination and also in media supplemented with TDZ+10% CM. Subsequent transfer of these BS onto shoot proliferation medium supplemented with BA (8.87 M) or -naphthalene acetic acid (NAA) (2.69 M) resulted in multiple shoot proliferation 17 ± 2.5 shoots and 30 ± 2.1 shoots respectively per explant. The multiple shoots so obtained were transferred to Nitsch medium (N69) containing BA (2.22 M) and gibberellic acid (GA3 ) (0.029 M) and also onto simultaneous shoot multiplication and root forming medium for further growth. For the first time the influence of zeatin, TDZ and coconut milk on shoot multiplication was studied. This protocol is effective in producing micropropagated vanilla plants with successful hardening and field transfer.

結果 (繁體中文) 1:

通過組織培養香莢蘭乘法是計算出與玉米素的協同活性，合成的細胞分裂素噻苯隆（TDZ）和椰奶（CM）的闡明新的線索以及N6芐基腺嘌呤（BA）。多個芽使用的Murashige和補充有玉米素斯庫格（MS）培養基，BA +玉米素，TDZ和TDZ +椰奶（CM）腋芽外植體顯影。外植體的性質和外植體接種到介質影響不僅多個拍攝生產方法，而且還球根枝芽（BS）的形成。玉米素支持多為單芽的生長，而BS的形成是在玉米素和BA組合在補充有TDZ + 10％CM培養基誘導和也。隨後的這些BS轉印到芽增殖培養基中添加BA（8.87 M）或萘乙酸（NAA）（2。69 M）分別導致多重芽增殖17±2.5芽和30±2.1芽每植體。如此獲得的多芽轉移到含有BA（2.22 M）NITSCH介質（N69）和赤黴酸（GA3）（0.029 M），並且還到同時枝條繁殖和進一步增長根形成介質。第一次玉米素，TDZ和枝條繁殖椰子汁的影響進行了研究。此協議是有效地產生微繁殖香草植物與成功的硬化和場傳遞。029 M），並且還到同時枝條繁殖和進一步增長根形成介質。第一次玉米素，TDZ和枝條繁殖椰子汁的影響進行了研究。此協議是有效地產生微繁殖香草植物與成功的硬化和場傳遞。029 M），並且還到同時枝條繁殖和進一步增長根形成介質。第一次玉米素，TDZ和枝條繁殖椰子汁的影響進行了研究。此協議是有效地產生微繁殖香草植物與成功的硬化和場傳遞。

結果 (繁體中文) 2:

High fever patients, Bs for the indications of CT chest and abdominal cavity, a large foci of infection are detected in the lungs. The current Bn is having to use prolonged antibiotics.

結果 (繁體中文) 3:

通過組織培養，研究了澤汀、合成細胞分裂素噻二唑侖（TDZ）、椰奶（CM）和N6苄基腺嘌呤（BA）的協同作用。採用Murashige和Skoog（MS）培養基，輔以玉米素、BA+玉米素、TDZ和TDZ+椰奶（CM），從腋芽外植體中培養出多個芽。外植體的性質和外植體在培養基上的接種管道不僅影響著多芽的產生，而且影響著球莖芽的形成。Zeatin主要支持單株的生長，而在Zeatin和BA的組合以及添加TDZ+10%CM的培養基中誘導BS的形成。隨後將這些BS轉移到添加BA（8.87m）或-萘乙酸（NAA）（2.69m）的芽增殖培養基上，每個外植體的芽增殖率分別為17±2.5和30±2.1。將獲得的多個芽轉移到含BA（2.22m）和赤黴素（GA3）（0.029m）的Nitsch培養基（N69）上，同時轉移到同時進行的芽增殖和生根培養基上繼續生長。首次研究了玉米素、TDZ和椰奶對芽增殖的影響。該協定是有效的生產微繁殖香草植物成功硬化和現場轉移。