Materials Required (and suggested Thermo Scientific Products)
• Wash Buffer: Tris buffered saline (TBS, Product No. 28376; 25 mM Tris, 150 mM NaCl, pH 7.2) with or without 1-2%
bovine serum albumin (BSA, Product No. 37520) and/or 0.05% Tween
®
-20 (Product No. 28320)
• Streptavidin or other biotin-binding protein: Streptavidin (Product No. 21122, 21125), NeutrAvidin Protein (Product No.
31000) or Avidin (Product No. 21121, 21128)
• Biotin: D-Biotin (Product No. 29129)