Acd is a well-known process most commonly used for chemicalpretreatment of the lignocellulosic biomass. Acid solubilises polysaccharides (especially hemicellulose) into monomers by enzymatichydrolysis leading to the enhancement of biofuel production. Thecommonly used conditions are low acid concentration with high temperature or high acid concentration with low temperature [48]. Themajor drawback of Acd is the production of various inhibitors (aceticacid, furfural and 5-hydroxymethyl furfural) which inhibits growth ofmicrobes