Cartilage was diced into 1 mm 3 pieces and digested overnight in 0.3% collagenase, 100 mg/m L penicillin, and 100 mg/m L streptomycin in Dulbecco’s modified Eagle’s medium (DMEM). The following day, the cells were filtered, washed, and counted.
Cartilage wasdiced into 1 mm3pieces and digested overnight in 0.3%collagenase, 100 mg/m L penicillin, and 100 mg/m L streptomycinin Dulbecco’s modified Eagle’s medium (DMEM). The followingday, the cells were filtered, washed, and counted.