The antifungal effects of Lactobaci

The antifungal effects of Lactobacillus casei AST18 on Penicillium chrysogenum. were investigated. Viable bacteria were used to detect the minimum inhibitory concentration of lactic acid bacteria (10
2 CFU/m L) to inhibit the growth of P. chrysogenum. Results showed that L. casei AST18 inhibited the growth of P. chrysogenum. The mycelial morphology was also altered. The scanning electron microscopy images of P. chrysogenum exposed to L. casei AST18 further showed that the mycelia were collapsed and appeared transparent. Numerous clots were also observed in the fungal cytoplasm. The tip of the hypha was deformed and exhibited a moniliform or in flated shape. The transmission electron microscopy images of L. casei AST18-exposed P. chrysogenum showed the occurrence of autophagy and death of fungal cells. Changes in the morphological characteristics of P. chrysogenum were observed in the antifungal activity experiment with viable bacteria, but such changes were not evident in the cell-free culture filtrate of L. casei AST18. This bacterial strain showed high antifungal activities and could be used as a fungal in-hibitor in food and medicine.

結果 (中文) 1:

对产黄青霉干酪乳杆菌 AST18 的抗真菌药物影响。进行了研究。活菌被用于检测的最低抑菌浓度的乳酸细菌 (102 CFU/m L) 以抑制 P.霉生长。结果表明，杆菌 AST18 P.霉生长有抑制作用。菌丝形态也有了变动。P.霉暴露 L.干酪乳杆菌 AST18 进一步表明，菌丝体崩溃出现透明扫描电子显微镜图像。众多的凝块也观察真菌的细胞质中。菌丝尖端变形和展出念珠状或在 flated 形状。L.干酪乳杆菌 AST18 暴露点霉透射电子显微镜图像显示细胞自噬的发生和真菌细胞的死亡。P.霉的形态特征观察抗真菌活性试验以活菌，但这种变化并不明显在无细胞培养 filtrate L.干酪乳杆菌 AST18。此菌株显示高的抗真菌活性，可以用作真菌在独联体食品和药品。

結果 (中文) 3:

干酪乳杆菌ast18对产黄青霉的抑菌作用。进行了调查。活菌进行乳酸菌的最低抑菌浓度（10
2 CFU / L）抑制产黄青霉生长。结果表明，干酪乳杆菌ast18抑制产黄青霉生长。菌丝形态也发生了改变。扫描电子显微镜图像的产黄青霉在干酪乳杆菌ast18进一步表明菌丝体倒塌，出现透明。真菌细胞质中也观察到大量的凝块。菌丝的前端变形，呈念珠状或fl了形状。透射电子显微镜图像的干酪乳杆菌ast18暴露P.产黄青霉表明自噬与真菌细胞死亡的发生。在与活菌的抑菌活性实验中观察到的产黄青霉的形态特征的变化，但这种变化并不明显，在干酪乳杆菌的无细胞滤液fiast18。该菌株具有较高的抗真菌活性，可以作为食品和药物抑制真菌。