不过，病毒载体有很多缺点，如制备困难、免疫原性、细胞毒性，因此，限制了

不过，病毒载体有很多缺点，如制备困难、免疫原性、细胞毒性，因此，限制了它在生命科学领域的应用

結果 (英文) 1:

However, viral vectors have many drawbacks, such as cytotoxicity preparation difficulties, immunogenicity, and therefore limited its application in life science field

結果 (英文) 2:

However, viral vectors have many drawbacks, such as difficulties in preparation, immunogenicity, cytotoxicity, thus, limiting its application in the field of life sciences

結果 (英文) 3:

However, viral vectors have many disadvantages, such as difficult to fabricate, immunogenicity, cell toxicity, therefore, restricted its application in life science field