CryoprotectantsCryoprotectants are compounds that depress the freezing temperature of foods, modify or suppress ice crystal growth during freezing and inhibit ice recrystallisation during frozen storage (see Section 22.3.2). They reduce damage to cell membranes and so protect the texture of foods and reduce the loss of nutrients in drip losses. Examples of cryoprotectants include sugars, amino acids, polyols, methyl amines, carbohydrates and inorganic salts (Kennedy, 2003). Cryoprotectant glycoproteins or ‘antifreeze proteins’ (AFPs) have been isolated from a wide variety of organisms, including bacteria (Kawahara, 2002), fungi, plants, invertebrates and fish, such as Antarctic cod and the winter flounder (Payne et al., 1994). The organisms have evolved AFPs as mechanisms to protect them against low temperatures. Multiple forms of AFPs are synthesised within each organism, each with a different function. For example, the ice nucleation protein acts as a template for ice formation, whereas the antinucleating protein inhibits ice nucleus formation at a foreign particle (see Section 22.1.1)(Kawahara, 2002). In future it may be possible to select an AFP with suitable characteristics and activity for particular food products and introduce it into the food by physical processes, such as mixing or soaking, or by gene transfer (Griffith and Ewart, 1995). AFPice complexes have interactions with cell membranes and with other molecules present in the solutions. Wang and Sun (2011), Wang (2000) and MacDonald and Lanier (2012) have reviewed studies of cryoprotectants, which show that AFPs have a complex mechanism of action and can display both protective and cytotoxic actions depending on the dose, type, composition and concentration of cryoprotectant, the characteristics of the biological material and the conditions of frozen storage.
Cryoprotectants<br>Cryoprotectants are compounds that depress the freezing temperature of foods, modify or suppress ice crystal growth during freezing and inhibit ice recrystallisation during frozen storage (see Section 22.3.2). They reduce damage to cell membranes and so protect the texture of foods and reduce the loss of nutrients in drip losses. Examples of cryoprotectants include sugars, amino acids, polyols, methyl amines, carbohydrates and inorganic salts (Kennedy, 2003). Cryoprotectant glycoproteins or ‘antifreeze proteins’ (AFPs) have been isolated from a wide variety of organisms, including bacteria (Kawahara, 2002), fungi, plants, invertebrates and fish, such as Antarctic cod and the winter flounder (Payne et al., 1994). The organisms have evolved AFPs as mechanisms to protect them against low temperatures. Multiple forms of AFPs are synthesised within each organism, each with a different function. For example, the ice nucleation protein acts as a template for ice formation, whereas the antinucleating protein inhibits ice nucleus formation at a foreign particle (see Section 22.1.1)(Kawahara, 2002). In future it may be possible to select an AFP with suitable characteristics and activity for particular food products and introduce it into the food by physical processes, such as mixing or soaking, or by gene transfer (Griffith and Ewart, 1995). AFPice complexes have interactions with cell membranes and with other molecules present in the solutions. Wang and Sun (2011), Wang (2000) and MacDonald and Lanier (2012) have reviewed studies of cryoprotectants, which show that AFPs have a complex mechanism of action and can display both protective and cytotoxic actions depending on the dose, type, composition and concentration of cryoprotectant, the characteristics of the biological material and the conditions of frozen storage.
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Cryoprotectants<br>Cryoprotectants are compounds that depress the freezing temperature of foods, modify or suppress ice crystal growth during freezing and inhibit ice recrystallisation during frozen storage (see Section 22.3.2). They reduce damage to cell membranes and so protect the texture of foods and reduce the loss of nutrients in drip losses. Examples of cryoprotectants include sugars, amino acids, polyols, methyl amines, carbohydrates and inorganic salts (Kennedy, 2003). Cryoprotectant glycoproteins or ‘antifreeze proteins’ (AFPs) have been isolated from a wide variety of organisms, including bacteria (Kawahara, 2002), fungi, plants, invertebrates and fish, such as Antarctic cod and the winter flounder (Payne et al., 1994). The organisms have evolved AFPs as mechanisms to protect them against low temperatures. Multiple forms of AFPs are synthesised within each organism, each with a different function. For example, the ice nucleation protein acts as a template for ice formation, whereas the antinucleating protein inhibits ice nucleus formation at a foreign particle (see Section 22.1.1)(Kawahara, 2002). In future it may be possible to select an AFP with suitable characteristics and activity for particular food products and introduce it into the food by physical processes, such as mixing or soaking, or by gene transfer (Griffith and Ewart, 1995). AFPice complexes have interactions with cell membranes and with other molecules present in the solutions. Wang and Sun (2011), Wang (2000) and MacDonald and Lanier (2012) have reviewed studies of cryoprotectants, which show that AFPs have a complex mechanism of action and can display both protective and cytotoxic actions depending on the dose, type, composition and concentration of cryoprotectant, the characteristics of the biological material and the conditions of frozen storage.
正在翻譯中..
