Gelfoam histoculture and growth medium
The vibrissae hair follicles were placed in DMEM-F12 (GIBCO/BRL, Grand Island, NY) containing B-27 (2.5%) (GIBCO/BRL), N2 (1%) (GIBCO/BRL), and 1% penicillin and streptomycin (GIBCO/BRL) on sterile Gelfoam (Pharmacia and Upjohn Co., Kalamazoo, MI) hydrated in the medium. The Gelfoam histocultured follicles were incubated at 37°C, 5% CO2 100% humidity. The medium was changed every other day [1, 8].