The aim of this work was to discove

The aim of this work was to discover if Mycoplasma fermentans, which is
known to infect B cells, could be the cause of the raised ecto-50-nucleotidase
observed in the synovial fluid of rheumatoid arthritis patients. The
ecto-50-nucleotidase activity in the patients’ serum has been shown to correlate
with the erythrocyte sedimentation rate and DNA from the mycoplasma
has been found in the synovial fluid. B lymphoblastoid cell lines
were exposed to 16 strains of Mycoplasma fermentans and their ecto-
50-nucleotidase, CD73, was measured both biochemically and by mouse
antibodies to human ecto 50-nucleotidase using the fluorescence activated
cell sorter. The type strain, PG 18, did not grow with the B cells. Some of
the mycoplasma strains (9/15) increased the cellular ecto-50-nucleotidase
activity from twice to 17 fold, and usually showed 50-nucleotidase activity
themselves. At least one strain, M106, induced human 50-nucleotidase on
the normally 50-nucleotidase negative Daudi and Raji Burkitt’s lymphoma
cell lines, and increased sevenfold the 50-nucleotidase on the monocyte/
macrophage cell line THP-1. Growing the cells in aged medium increased
the level of mycoplasma infection. This mycoplasma-induced enzyme
showed a conformational change and an increase in activity with a glycosylation
change involving mannose groups. The other group of strains,
mostly of respiratory or cell culture origin, usually did not have any
50-nucleotidase of their own and decreased the B-cell enzyme activity by
about half. Electron microscopy and flow cytometry showed that the
strain M106 was filamentous and could be found inside the B-cells. The
50-nucleotidase-inducing strains of M. fermentans may be important in
the aetiology of rheumatoid arthritis

結果 (中文) 1:

这项工作的目的是如果发现发酵支原体，是已知感染 B 细胞，可能是凸起的外 50 核苷酸的原因类风湿性关节炎患者的滑膜液中观察到。的病人的血清外-50-核苷酸酶活性已被证明关联与红细胞沉降率和 DNA 从支原体滑膜液中发现。B 淋巴母细胞系被暴露了 16 株发酵支原体和其外.50-核苷酸酶 CD73，测定生化和鼠标人类外 50-核苷酸酶荧光激活抗体电池分选机。型菌株中，PG 18 与 B 细胞并没有增长。一些支原体株 （9/15) 增加细胞的外 50 核苷酸从两倍到 17 倍和通常显示的 50-核苷酸酶活性自己。至少一个应变，M106，诱导人类 50-核苷酸酶通常 50-核苷酸酶阴性达乌迪和 Raji 伯基特淋巴瘤细胞株，并增加了 7 倍 50-核苷酸酶对单核细胞 /巨噬细胞株 THP 1。增加老年介质中生长的细胞支原体感染水平。这种肺炎支原体诱导的酶显示构象的改变和增加与糖基化的活动更改涉及的甘露糖组。另一组的菌株，主要的呼吸或细胞培养起源，通常没有任何50-核苷酸酶的自己和降低由 B 细胞酶活性大约一半。电子显微镜和流式细胞仪表明应变 M106 呈丝状，可以发现 B 细胞内。的M.发酵 50 核苷酸诱导株可能是重要的类风湿性关节炎的病因

結果 (中文) 3:

这项工作的目的是发现，如果发酵支原体，这是
已知感染B细胞，可提高ecto-50-nucleotidase
在类风湿关节炎滑膜液观察的原因。的
ecto-50-nucleotidase活动在患者血清中已经显示出相关的
与红细胞沉降率和DNA的
支原体已在滑液的发现。B淋巴母细胞系
被暴露在16株发酵支原体和外生
50核苷酸酶，CD73，测定生化和鼠标
抗体体外50核苷酸酶利用荧光激活细胞分选
。型菌株，PG 18，没有与B细胞的生长。一些
支原体菌株（9 / 15）的细胞ecto-50-nucleotidase
活性增加两倍至17倍，通常表明50核苷酸酶活性
自己。至少一个应变，M106，诱导50核苷酸酶在
通常50核苷酸酶阴性Burkitt淋巴瘤raji与daudi细胞系和
，增加了7倍的50核苷酸酶对单核细胞/巨噬细胞系THP-1
。在老年人中增加
支原体感染水平的细胞生长。这
表明支原体引起的酶构象变化与涉及甘露糖组糖基化
改变活动的增加。菌株的其他组，
大多呼吸或培养细胞的起源，通常没有任何
50核苷酸酶和自己减少B细胞的酶活性的
大约一半。电子显微镜和流式细胞仪检测表明，
应变M106呈丝状，可以在B细胞的发现。的
50核苷酸酶诱导M.发酵菌株可能
类风湿性关节炎的病因是很重要的