The next day, membranes were washed

The next day, membranes were washed and incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies at room temperature for 1 h. Antibodies were detected with enhanced chemiluminescence substrate

結果 (繁體中文) 1:

第二天，洗滌膜，並用辣根過氧化物酶（HRP）綴合的孵育的第二抗體在室溫下1小時。用增強的化學發光底物檢測抗體

結果 (繁體中文) 2:

第二天，用馬蘿蔔過氧化物酶（HRP）結合的二級抗體在室溫下清洗和孵育膜1小時。用增強的化學發光基質檢測抗體

結果 (繁體中文) 3:

第二天，在室溫下用辣根過氧化物酶（HRP）結合的二級抗體清洗和孵育1h。用增强化學發光底物檢測抗體