OC obtained from the above-mentioned co-culture were transferred into either (i) 24-well culture plates, (ii) dentine slices (6 mm in diameter; MS Labo System, Yokohama, Japan) in 24-well culture plates, or (iii) a calcium phosphate-coated plate in 24-well plates, and incubated in the presence of MBE (0–333 μg/mL) for a further 24 h.