Dermal fibroblasts were seeded on 60-mm plates and incubated for 1 day. And, cells were irradiated with UVA 6 J/cm2 and then incubated overnight in Iscove’s modified Dulbecco’s medium containing 10% FBS and RG NG or RG WE (0, 100, 250, or 500 mg/ml). The conditioned media were collected using an Amicon Ultra-4 centrifugal filter (Millipore, USA). The proteins in the media were concentrated by centrifugation (13,000 g for 3 h at 4C). The proteins were separated on 10% zymogram gel (Invitrogen, USA), and the gels were renatured in 2.5% Triton X-100 and then were developed in buffer with 50 mM Tris-HCl, pH 7.6, 1 mM CaCl2, 0.2 M NaCl overnight at 37C. The gels were stained with Coomassie blue (Brilliant blue G solution; Sigma, USA) and destained in buffer with 10% acetic acid and MeOH. Gelatinolytic activity, i.e., unstained areas on gel, was quantified by using Luminograph II and CSAnalyzer4 program (Atto, Japan). Zymography was performed in triplicates for each sample.