The antibacterial activity of dichloromethane and chloroform fractions of methanol extract were screened by the agar well dif- fusion method (Mohsen Abolhassani 200)) against 24 clinical iso- lates of each of eight bacterial strains belonging to Gram-positive Staphylococcus aureus and Gram-negative Klebsiella pneumonia, and Pseudomonas aeruginosa (Table 1). The bacterial strains used for screening antimicrobial activity were collected from different infectious statuses of patients who had not taken any antibacterial drugs for at least two weeks with the help of an authorized physi- cian, in the district health center of Gulberga, Karnataka State, India. The clinical isolates were identified following a standard method (Cown et al. 1993). The bacterial suspensions were diluted in 10-1 to 10-8 phosphate buffered saline. Samples were homo- genized and then loaded in six aliquots of 20 L each onto nutrient agar plates (agar 15 g/L, beef extract 1 g/L, peptone 5 g/L, NaCl 5
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