Sequencing was performed using a Bi

Sequencing was performed using a Big Dye Terminator Ready Reaction Mix v1.1 kit and primers described in Supplementary Material, Table S3. Data were collected using an Applied Biosystems 3130xl Genetic Analyser. Data were analysed by visual inspection of electropherograms and using Mutation Surveyor software (SoftGenetics, Inc.).
For 78 of 79 tumour samples containing variants, the variant was confirmed in a second PCR using DNA that had not been whole-genome-amplified. One variant (tumour 1135) was confirmed in a second PCR from whole-genome-amplified DNA. Somatic mutation status in tumours was confirmed by analysis of normal matched blood samples except for samples 271, 595, 991, 1294, 1415 and 1434.

結果 (中文) 3:

进行测序，使用一个大的染料终止准备反应混合V1.1试剂和引物 补充材料描述，表S3。数据均用一个ABI 3130xl遗传分析仪收集。数据由电泳分析和利用视觉检测突变测量软件（softgenetics
，Inc.）。78 79肿瘤样本含有变种，不同的是使用DNA没有全基因组扩增PCR证实二。一个变量（肿瘤1135）在全基因组DNA扩增PCR证实第二。在肿瘤中的体细胞突变状态的分析证实，正常的血液样本相匹配除了样品271，595，991，1294，1415和1434。