Native or modified lecithins are widely used as a multifunctional ingredient in the food industry. A fractionation process of sunflower lecithin (a non GMO product) with absolute ethanol was used for obtaining enriched fractions in certain phospholipids under different experimental conditions (temperature 35–65 °C, time of fractionation 30–90 min, ethanol/lecithin ratio 2:1, 3:1). Phospholipid enrichment in PC and PI fractions was obtained and analyzed by 31 P NMR determinations. The percent extraction coefficients for different phospholipids (%E PC , %E PE and %E PI ) in both fractions were calculated. Values of %E PC in PC fractions significantly increased ( p < 0.05) from 12.8 (35 °C, 30 min, 2:1) to 57.7 (65 °C, 90 min, 3:1) at increasing temperature and incubation time. %E PE varied from 3.0 to 18.3 in the same fraction while %E PI presented lower values (