For isotherm determination, 2.0-g of air-dried, homogenized soil was treated with 20 mL of 0.01 M KCl solution containing 0, 0.1, 1, 5, 10, 25, 50, and 100 mgP L−1 (Graetz and Nair, 1995; Nair et al., 1998) in 50 mL centrifuge tubes. The tubes were placed on a mechanical shaker for a 24 h equilibration period. At theend ofthe equilibration period, samples were centrifuged at 6000 rpm for 10 min and the supernatant was filtered through aFig. 2. Soil sampling locations for Spodosol (Lake Okeechobee Basin) and Ultisol (Suwannee River Basin) soil profiles. In addition, 80 Bh and 28 Bt horizon samples were included for cross-validation of the predictive equations.B. Dari et al. Agriculture, Ecosystems and Environment 245 (2017) 92–99940.45μm membrane filter. The filtrate was analyzed for soluble reactive P by colorimetric method (Murphy and Riley, 1962) using a Technicon™ Autoanalyzer (EPA 365.1).
For isotherm determination, 2.0-g of air-dried, homogenized soil was treated with 20 mL of 0.01 M KCl solution containing 0, 0.1, 1, 5, 10, 25, 50, and 100 mgP L−1 (Graetz and Nair, 1995; Nair et al., 1998) in 50 mL centrifuge tubes. The tubes were placed on a mechanical shaker for a 24 h equilibration period. At theend ofthe equilibration period, samples were centrifuged at 6000 rpm for 10 min and the supernatant was filtered through a<br>Fig. 2. Soil sampling locations for Spodosol (Lake Okeechobee Basin) and Ultisol (Suwannee River Basin) soil profiles. In addition, 80 Bh and 28 Bt horizon samples were included for cross-validation of the predictive equations.<br>B. Dari et al. Agriculture, Ecosystems and Environment 245 (2017) 92–99<br>94<br>0.45μm濾膜濾波器。對FI ltrate分析通過比色法(墨菲和萊利,1962)使用的Technicon™自動分析儀(EPA 365.1)可溶於活性磷。
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