Subculture and rapid propagation
After inoculation,the generated callus or multiple bud clumps were transferred to MS + 0 - 4. 0 mg/L 6-BA + 0 - 0. 4 mg/L NAA for subculture and rapid propagation. There are two ways for subculture of H. hybrida,which can be propagated rapid- ly by cutting the callus during callus stage or separating the multiple bud clumps.