A 46% reduction in LT50 and 68% in LT90 was obtained when the Bv062 conidia suspension (1x106 con/mL) was supplemented with 300 µg/mL recombinant chitinase, compared to using just fungal conidia. This result suggested that rChit37 improved Bv062 conidia virulence against second instar larvae of D. saccharalis, reducing doses and lethal times. Such reduction could have been attributed to rChit37 chitobiosidase and endochitinase activity considering that it has been demonstrated that the chitinolytic activity of entomopathogenic fungi such as B. bassiana and M. anisopliae is directly related to their virulence
正在翻譯中..