Compared to the PF of around2.0 which we achieved for the recombinant HRP isoenzyme C1A using SEC before [33], the flowthrough step applying an anion exchange monolith presented here is not only advantageous in terms of flow rates,sample volumes and thus process time, but also gave a 5-fold higher PF of nearly 11.0. In Fig. 3 we exemplarily show a SDS gel of the different steps during AEC purification of the HRP isoenzyme C1A.