Thank you for contacting CST technical support regards to our Atg5 siRNA (#6345) and Atg7 siRNA (#6004). I'm sorry to hear you're experiencing issues with knockdown in your samples. Vendor protocols can vary greatly depending on which transfection reagent you choose, so it's a little more difficult to troubleshoot through that process because it's not from CST. I'd be happy to send you one of our conjugated control siRNAs:#86921 SignalSilence® Control siRNA (Cy5® Conjugate) #78193 SignalSilence® Control siRNA (Cy3® Conjugate) #6201 SignalSilence® Control siRNA (Fluorescein Conjugate) This way you can visualize transfection efficiency in your cells to ensure the transfection process is working. If you'd like to continue troubleshooting, I could provide a vial of this control, as well as an additional vial of either Atg5 or Atg7 for testing?If you are interested, please include your shipping address so I can process these samples for you. I look forward to hearing back!