A. Compound Ia (0.7183 g., 0.002 mo

A. Compound Ia (0.7183 g., 0.002 mole) was dissolved in 30 ml. of 0.2 N. sodium hydroxide, and the solution was kept under nitrogen. At time intervals aliquots of the solution were acidified
with acetic acid and titrated with iodine. Five minutes after dissolution 66% of the S-benzoyl group was removed; after 15 minutes almost l00%. The rest of the solution was acidified with sulfuric acid, and after oxidation with iodine the solution was extracted with ethyl acetate. The ethyl acetate layer was washed with water until the water extract became neutral to congo red paper, dried over sodium sulfate and concentrated to a small volume in vacuo. Upon the addition of cyclohexylamine to the solution, N,N"-biscarbobenzoxy-L-cystine cyclohexylamine salt separated. The yield of product recrystallized from ethanol was 35%, calculated on the basis of the amount of Ia used; m.p. 183 oC (reported183 oC).
B. Compound Ia (0.001 mole) was dissolved in 20 ml. of aqueous 2 N ammonia. The solution was kept under nitrogen and was worked up as in case A. Fifteen minutes after dissolution, 51% of the S-benzoyl group was split off; after 30 minutes, 95% was removed.
Saponification of the S-acetyl group of Id by procedure A as described for Ia was almost complete after 2 minutes; for a complete ammonolysis of the S-acetyl group (procedure B) approx. 12 minutes was required.

結果 (中文) 1:

A.复合 Ia (0.7183 g.，0.002 鼹鼠) 被溶化在 30 毫升。 0.2; 氢氧化钠、 和解决方案受到氮。在时间间隔整除数的解决方案被酸化以乙酸和用碘滴定。五分钟后溶出 66%的 S-苯甲酰基组被删除;15 分钟几乎 l00%之后。其余的解决方案被酸化与硫酸，以及氧化碘液用乙酸乙酯提取后。乙酸乙酯层直到水提取物成为中性至刚果红试纸，干过硫酸钠和在真空中集中到一个小卷，用水洗净。加入了环己胺到解决方案中，N，N 后"-biscarbobenzoxy-L-胱氨酸环己胺盐分离。用乙醇重结晶的产物收率为 35%，计算所用的 Ia 使用;业主立案法团阁下 183 (reported183 oC)。B.复合 Ia (0.001 摩尔) 被溶化氨。 在 20 毫升。 水 2 N 的该解决方案受到氮和动情，在案例 A.十五分钟后解散，51%的 S-苯甲酰基组被分裂了;30 分钟后，95%的被删除。S-乙酰基组的 Id 的过程 A Ia 为所述皂化后 2 分钟; 几乎完全完整的氨解 S-乙酰基组 (程序 B) 约 12 分钟就需要。

結果 (中文) 3:

化合物为0.7183克，0.002毫升，0.2毫升的化合物，在30个N。在时间间隔等分的溶液进行酸化
乙酸滴定碘。之后的s-benzoyl组解散66%辗转五分钟；15分钟后几乎100%。该解决方案的其余部分是用硫酸酸化，并在氧化后用碘溶液萃取乙酸乙酯。乙酸乙酯层，用水洗至中性水提取物成为刚果红纸，干燥硫酸钠和真空浓缩至小体积。在溶液中加入环己胺，N，N“- biscarbobenzoxy-l-cystine环己胺盐分离。产品从乙醇重结晶收率为35%，对IA的金额计算；熔点183℃（reported183 OC）。
B.复合IA（0.001摩尔）溶解在2毫升水氨氮20。解决方案是在氮和曾作为案例A十五分钟溶解后，51%组的s-benzoyl分裂；30分钟后，除去95%
。通过程序所描述的是几乎完整的2分钟后皂化ID的S-乙酰基组；一个完整的氨解的S-乙酰基组（方法B）约12分钟是必需的。