310. Radiat Res. 2002 Feb;157(2):19

310. Radiat Res. 2002 Feb;157(2):191-8.

Role of CD13/aminopeptidase N in rat lymphocytic alveolitis caused by thoracic
irradiation.

Huang L(1), Tani K, Ogushi F, Ogawa H, Shimizu T, Motoki Y, Moriguchi H, Sone S.

Author information:
(1)Third Department of Internal Medicine, School of Medicine, Tokushima
University, Tokushima, Japan.

CD13/aminopeptidase N is a cell surface glycoprotein that is widely distributed
in a variety of mammalian cells. It was recently shown to have chemotactic
activity for T lymphocytes. This study examined the role of CD13/aminopeptidase N
in lymphocytic alveolitis in radiation-induced lung injury caused by a
single-dose thoracic irradiation (15 Gy) in rats. Significantly increased
aminopeptidase activity was detected in bronchoalveolar lavage fluid obtained
from irradiated rats at 4 weeks after irradiation compared to the activity in
unirradiated rats. Significantly higher aminopeptidase activity was detected on
alveolar macrophages from irradiated rats at 2 and 4 weeks than on those from
unirradiated rats. Western blot analysis showed an increased expression of
CD13/aminopeptidase N protein in alveolar macrophages from irradiated rats at 4
weeks. Chemotactic activity for normal rat lymphocytes was detected in
bronchoalveolar lavage fluid from irradiated rats at 4 weeks, and approximately
60% of the activity was inhibited by pretreatment of bronchoalveolar lavage fluid
with bestatin, a specific aminopeptidase inhibitor. This study suggests that
CD13/aminopeptidase N may play an important role as a lymphocyte chemoattractant
in lymphocyte-mediated alveolitis in experimental radiation-induced lung injury.

PMID: 11835683 [PubMed - indexed for MEDLINE]

結果 (中文) 1:

310.辐射 res.2002 Feb; 2:191 8。CD13/氨肽酶 N 在大鼠淋巴细胞性肺泡炎致胸中的作用照射。黄 L(1)，Tani K，小栉 F、 小川 H、 清水 T、 诚记 Y，守口 H，一些美国作者信息︰(1)第三部门的医学院，内科德岛德岛县，日本大学。CD13/氨肽酶 N 是细胞表面糖蛋白，广泛分布在哺乳动物细胞的各种行动。它最近被证明有趋化T 淋巴细胞的活性。本研究考察了 CD13/氨肽酶 N 的作用在淋巴细胞性肺泡炎致辐射引起的肺损伤单剂量胸部照射 (15 Gy) 大鼠。显著增加氨肽酶活性检测在支气管肺泡灌洗液获得从辐照大鼠 4 周后照射比较中的活动未受辐照的大鼠。高氨肽酶活性显著上检测到在 2 周和 4 周比那些从辐照大鼠肺泡巨噬细胞未受辐照的大鼠。免疫印迹分析表明的表达增加CD13/氨肽酶 N 蛋白 4 在辐照大鼠肺泡巨噬细胞周。对正常大鼠淋巴细胞趋化活性检测支气管肺泡灌洗液从辐照大鼠 4 周，和约60%的活性被抑制的支气管肺泡灌洗液的预处理与乌苯美司，一种特定氨基肽酶抑制剂。这项研究表明，CD13/氨肽酶 N 可能发挥重要作用作为淋巴细胞趋化因子在淋巴细胞介导的肺泡炎辐射诱导的实验性肺损伤。PMID: 11835683 [PubMed-索引为 MEDLINE]

結果 (中文) 3:

310。辐射研究。2002年2月；（2）：157—8 191。角色的淋巴细胞在大鼠CD13（氨肽酶N模式alveolitis所致胸辐照。（1）、黄谷ogushi K，F，H T小川，守口清水，于Y，H，S的人。作者信息：（1）三内部医学部，医学部徳岛大学，日本。CD13（氨肽酶N is that is a细胞表面糖蛋白广泛分布在mammalian学院在各种细胞。它recently shown to have趋化T for lymphocytes活性。本研究examined CD13（氨肽酶N«在淋巴细胞中的辐射诱导肺损伤alveolitis带给by a胸照射单剂量（15戈瑞）在大鼠。significantly increased氨肽酶的活性是在支气管肺泡液obtained lavage detected从大鼠4周后照射在辐照compared to the”unirradiated鼠。significantly氨肽酶活性高是detected在线alveolar macrophages从辐照AT 2和4周大鼠比那些从在线unirradiated鼠。Western blot分析给安increased expression ofCD13（氨肽酶N蛋白在大鼠alveolar macrophages从辐照4。周。趋化活性在大鼠detected lymphocytes was for normal在辐照大鼠支气管肺泡lavage流体从4周，和相角近似为抑制活性的60% of the of lavage支气管流体的预处理模式与氨肽酶抑制剂bestatin，特异性。本研究suggests that氨肽酶N播放/安CD13可能重要的角色在淋巴细胞趋化。在实验中的淋巴细胞介导的alveolitis辐射诱导肺损伤。pmid：PubMed MEDLINE indexed for 11835683 [ - ]