The DPPH (2,2-diphenyl-1-picrylhydrazyl) method was used to measure the antioxidant activitywith some modifications [64]. Having found very similar results between methanol and bufferedmethanol, the DPPH solution was prepared by mixing methanol with DPPH reagent (Sigma Aldrich,Sigma-Aldrich, Dorset, UK) at a molarity of 100 µM to ensure maximum absorbances of around 1.0.For the analysis, 200 µL of the extracts (in triplicate) were mixed with 2 mL of the DPPH solution.The mixture was incubated for 30 min in the dark and the absorbance was measured at 517 nm using aspectrophotometer (Thermoelection Corp., Nottingham, UK). The antioxidant activity values wereexpressed as the percentage of absorbance change, by comparing the absorbance of individual samplesagainst that of the control (200 µL of methanol +2 mL of DPPH reagent).