Experimental study on different concentration of polysorbate 80 on zebrafish tissue homogenate of glutathione reductase (GR) effects. Experiments can be divided into 5 groups, 0.0016, 0.008, respectively, 0.04, 0.2 g/l concentration gradient Tween 80 groups and water control, continuous processing 1d, 3D, 7d, 14d, respectively. Experiment results showed that: spit temperature 80 Qian two group and to ion water control group compared GR content first declined Hou increased, but are below control group content; third group GR content first increased Hou reduced, in concentration for 0.0016g/L, and Shi, GR content highest, Hou declined and are below control group GR of content; fourth group, GR content first reduced Hou increased again reduced, and in 0.04g/L Shi concentration highest。 Conclusion a certain concentration of polysorbate 80 GR content was reduced in time, with the extension of time, different time on GR activity promotes individual concentration.
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