Dilution: Monoclonal Mouse Anti-Human C5b-9, code No. 0777, may be used at a dilution range of 1:25-1:50
when applied on acetone-fixed cryostat sections of tonsil or kidney and using 30 minutes incubation at room
temperature with the primary antibody. Optimal conditions may vary depending on specimen and preparation
method, and should be determined by each individual laboratory. The recommended negative control is
DakoCytomation Mouse IgG2a, code No. X 0943, diluted to the same mouse IgG2a concentration as the
primary antibody.
Visualization: DAKO LSAB+/HRP kit, code No. K 0679, and DAKO EnVision+/HRP kits, code Nos. K 4004
and K 4006, are recommended. For frozen sections and cell preparations, DakoCytomation APAAP kit, code
No. K 0670, is a good alternative if endogenous peroxidase staining is a concern. Follow the procedure
enclosed with the selected visualization kit.