Frz is the recently developed novel approach for physical pretreatment of biomass and has ability to significantly increase the enzymedigestibility of lignocellulosic biomass. The method being very costintensive and hence not much work has been done on it. The methodhas unique features like application of less dangerous chemicals, lowernegative environmental impact and high productiveness. Chang et al.[112] reported 48–84% increment in enzyme digestibility of rice strawusing Frz with 417.27 g/kg and 138.77 g/kg reducing sugar yields forPretreatments of feedstock used for Bio-methane production.150 U cellulase and 100 U xylanase for 48 h respectively. Smichi et al.pretreated Juncus maritimus with Frz/thawing pretreatment forits conversion to bioethanol by Saccharomyces cerevisiae. Theoreticalethanol yield was (84.28 ± 5.11) % with a productivity yield of(0.88 ± 0.16) g L−1 h−1 which was (90.87 ± 1.94) % of the theoretical yield. Freezing is also used to dry the lignocellulosic biomass