Construction of recombinant plasmids pFastpVP2 and
recombinant bacmid
The recombinant plasmids pFastpVP2 and B-pFastpVP2
were identified by BamHI and SalI enzyme digestion.
The fragments were about 1800 bp and 4775 bp, respectively, which conformed to the expected sizes (Figure 1).
Recombinant bacmid was identified by PCR with the
primers PPV-VP2 FD and PPV-VP2 FD. The fragment
was about 1840 bp.