Preparation of Stock Solution: Standard stock solution of
olopatadine hydrochloride was prepared by dissolving 10mg of
olopatadine hydrochloride in 10ml of methanol and 0.1N HCl
(50:50) to produce a concentration of 1000μg/ml. 1ml of this stock
solution was taken and then diluted up to 10ml by using methanol
and 0.1N HCl (50:50) to produce a concentration of 100μg/ml which
is the standard stock solution.