CHAPTER 2 Acute toxicity of copper, lead, zinc and their mixtures on
Evechinus chloroticus and Haliotis iris larvae ............................................................ 17
2.1 Introduction ........................................................................................................... 18
2.2 Methods ................................................................................................................ 21
2.2.1 Broodstock and spawning .............................................................................. 21
2.2.2 Larval assays ................................................................................................. 23
2.2.3 End point ........................................................................................................ 24
2.2.4 Statistics ......................................................................................................... 28
2.3 Results ................................................................................................................... 29
2.3.1 Single metals .................................................................................................. 30
2.3.2 Metal mixtures ............................................................................................... 38
2.4 Discussion ............................................................................................................. 39
2.4.1 Comparison with current water quality criteria ............................................ 40
2.4.2 Comparison with related species ................................................................... 40
2.4.3 Effect of metals on larval development .......................................................... 45
2.4.4 Metal mixtures ............................................................................................... 47
2.4.5 Consequence for field populations ................................................................ 48
2.4.6 Conclusion ..................................................................................................... 49
CHAPTER 3 Latent effect of low level of copper contamination on Evechinus
chloroticus early life stages ........................................................................................... 51
3.1 Introduction ........................................................................................................... 52
3.2 Methods ................................................................................................................ 55
3.2.1 Broodstock and spawning .............................................................................. 55
3.2.2 Metal concentrations ..................................................................................... 55
3.2.3 Larval assays ................................................................................................. 56
3.2.4 Settlement ....................................................................................................... 60
3.2.5 Juvenile re-exposure experiment ................................................................... 61
3.2.6 Statistics ......................................................................................................... 62
3.3 Results ................................................................................................................... 65
3.3.1 Larval survival, growth and development ..................................................... 65
3.3.2 Settler performance ....................................................................................... 65