The HPLC separation was also performed using the aboveequipment with an AQ-C18 (250 mm 4.6 mm, 5 mm)column at a column temperature of 251C and liquid flowrate of 1.0 mL/min using (A) 0.2% aqueous formic acid and(B) acetonitrile as mobile phase with the flow-rate of 1.0 mL/min using a gradient elution of 35% B at 0–10.0 min,35–50% B at 10.0–20.0 min, 50% B at 20.0–30.0 min,50–95% B at 30.0–50.0 min.