Using aseptic technique, weigh 50 g of sample into sterile blender jar. Add 450 mL Butterfield's phosphate-buffered dilution water (1:10 dilution) and blend for 2 min at high speed (10,000-12,000 rpm). Prepare serial dilutions from 10-2 to 10-6 by transferring 10 mL homogenized sample (1:10 dilution) to 90 mL dilution blank, mixing well with vigorous shaking, and continuing until 10-6 dilution is reached.
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