with nutrient absorption (Lajolo and Genovese, 2002). Salmonids fed
soy products have exhibited intestinal damage similar towhatwould be
expected from SBA consumption (van den Ingh et al., 1991; Burrells
et al., 1999; Krogdahl et al., 2003). However, there have been few
evaluations of purified sources of lectin.Hendricks et al. (1990) reported
purified SBA binds in vitro to both proximal and distal preparations of
the brush border membrane in Atlantic salmon. Buttle et al. (2001)
reported significant histological changes in Atlantic salmon fed 3.5%
dietary SBA, but only in the distal segments of the gastrointestinal tract.
They also reported similar results in rainbow trout fed a diet containing
60% SBM, but did not evaluate a purified source of SBA in trout. Iwashita
et al. (2008) fed trout purified diets containing 0.0075% SBA and clearly
identified that ANF as contributing to enteritis in the distal segment of
the gastrointestinal tract. However, there were no changes in weight
gain in trout fed SBA. Subsequently, Iwashita et al. (2009) identified
both saponin and SBA as causative agents in distal enteritis in trout fed
purified diets. Processed SBM typically contains 10–200 mg/kg SBA
(Russett, 2002); thus, the concentration evaluated in salmon was well
above what would be expected in SBM, while the concentrations
evaluated by Iwashita et al. (2008) in trout were within the range that
would be expected.