Refstie et al. (1998) reported thatweight gain in Atlantic salmon fed
a SBM containing reduced concentrations of TI, SBA and OLIG was not
significantly different fromfish fed a fishmeal control diet.However, the
study was not designed to evaluate interactive effects of ANF. Knudsen
et al. (2008), using a crude ethanol extract from soybeans, concluded
that both soy saponins and an unidentified compound were likely
contributors to enteritis in salmon. The published data on soybeanmeal
use in trout clearly indicates negative effects atmoderate to high levels
of inclusion in diets, yet the factor(s) causing this limitation remain
unclear and there are no indicators of those effects.
The goal of this study was to take a more rigorous approach in
experimental dietary formulation using purified ANF and test the
hypotheses that: (1) soybean ANF were not causing impacts on weight
gain of trout; and, (2) interactive effects of soybean ANF were not
influencing weight gain in trout.