3.3. Separation and purification of

3.3. Separation and purification of oligosaccharides
Chromatography of enzymatic hydrolysate by using tandem columns is shown in Fig. 2A. Four major fractions which possesse significantly high RID signals were nominated as fraction I, II, III and IV. The distribution coefficients of those four fractions were 0.416, 0.443, 0.619 and 0.674. Referring to the oligosaccharide profile and corresponding distribution coefficients determined by LC–MS, fraction I, II, III and IV were predicted to be disulphated trifucose, disulphated tetrafucose, tetrasulphated heptafucose and tetrasulphated octafucose, respectively. Those oligosaccharides were separated and selected for further structural analysis. The yields of fraction I, II, III and IV were 8.8%, 8.3%, 8.6% and 7.6%, respectively. Their purities were identified by FACE, as shown in Fig. 2B. The band of each purified oligosaccharide was narrow and distinct, and furthermore there was no miscellaneous band around. This demonstrates that the purities of the oligosaccharides were high.

結果 (中文) 1:

3.3.分离和低聚糖 purification酶法水解液，采用串联柱色谱法如图 2A 所示。四个主要组分的清谈有力地高 RID 信号被提名为分数一、 二、 三和四。那些四种蛋白组分的分布系数被 0.416、 0.443、 0.619 和 0.674。指的是低聚糖形和相应由 LC — — MS，一、 二、 三和四是的分数的分布系数预测分别为 disulphated trifucose、 disulphated tetrafucose、 tetrasulphated heptafucose、 tetrasulphated octafucose。这些低聚糖分离并进行了结构分析。收益率的部分一、 二、 三和四分别 8.8%、 8.3%、 8.6%和 7.6%。它们的纯度甚至是种鉴定的脸，如图 2B 所示。每个净化低聚糖的乐队又窄又分明，还有此外周围没有杂项乐队。这说明低聚糖的纯度较高。

結果 (中文) 3:

3.3。采用串联柱分离纯化fi阳离子低聚糖
色谱法酶解产物如图2a所示。四大组分具有明显fi明显高信号被提名为摆脱部分I，II，III和IV的分布系数fi系数这四个分数分别为0.416，0.443，0.619和0.674。指的是寡糖亲fi乐和相应的分配系数fi系数由LC–MS，确定部分I，II，将disulphated trifucose，disulphated tetrafucose是III和IV，tetrasulphated heptafucose和tetrasulphated octafucose，分别。这些低聚糖的分离和选择用于进一步的结构分析。我的分数产生，二，III和IV分别为8.8%，8.3%，8.6%和7.6%，分别。其纯度进行鉴定fi的脸，如图2b所示。每个普瑞fiED寡糖带很窄的和明显的，而且没有杂带。这表明，低聚糖的纯度很高。