Precision
Precision of the study was tested at three different levels.
The first consisted of checking instrumental system precision,
where a sample corresponding to concentration of 50μg/mL was
performed six times consecutively into the system, repeating the
operation once again the next day. The second test consisted of
checking the precision of the method, operating as described
in the standard solution: six individual samples were prepared
and the RSD was studied for the peak area obtained. Third,
the intermediate precision was studied by preparing samples
according to the precision of method and studying variability in
the method when it is used by different analysts on different days.