The use of different types and concentrations of organic acids ascoagulant resulted in textural and microstructural differences intofu. Rheological properties, pH, and in-gel chemical interactionssuggested that accelerated acidification reduces the amount of timeavailable for ordered aggregation of soy proteins, thus loweringtofu quality.Tofu acidified with 0.14 g/100 mL organic acids exhibitedexcellent physical properties, as indicated by high storage modulus,hardness,water-holding capacity, and non-freezablewater content.In addition, these gels were formed mostly because of hydrophobicinteractions. On the other hand, tofu prepared with 0.12 and 0.16 g/100 mL citric acid were comparable in hardness, although theformer was more elastic. Similar trends were observed for tofuprepared with 0.12 and 0.16 g/100 mL L-(-)-malic acid. However,tofu prepared with tartaric acid had comparatively poorer physicalproperties and less robust chemical interactions. Finally, tofu preparedwith 0.18 g/100 mL organic acid was of low quality, as indicatedby low elastic modulus, hardness, water-holding capacity,non-freezable water content, and coarse gel structure. Collectively,the data indicated that the quality of tofu prepared withorganic acids is comparable to that prepared with GDL. Our studyrevealed that different types tofu (soft or firm) can be produced in astraightforward manner by using suitable types and concentrationsof organic acids.