On d 60 of lactation, biopsies of t

On d 60 of lactation, biopsies of the mammary glands of 8 goats were also performed after challenge with ACTH or the placebo. The 8 goats were randomly distributed, with 4 in each treatment. Each biopsy was performed 30 min after the administration of ACTH or the placebo. After trichotomy, antisepsis, and anesthetic procedures, the skin incision was performed at the medial part of the gland with a scalpel. The disposable biopsy needle was inserted into the incision to a depth of approximately 3.0 cm, and the equipment was triggered to cut and collect a sample of approximately 2.0 × 0.5 cm of mammary tissue. Three tissue samples were collected. The first was collected to use in determining the rates of apoptosis and proliferation, the second was used for the analysis of gene expression by reverse-transcription (RT) PCR, and the third was used for cell cultures and the subsequent administration of in vitro treatments.

結果 (繁體中文) 1:

對哺乳的d 60，8山羊乳腺活檢也與ACTH或安慰劑挑戰之後進行。8山羊每個處理被隨機分佈，具有4。每個活檢ACTH或安慰劑的給藥後30分鐘進行的。三分法，防腐和麻醉程序後，皮膚切口處用解剖刀腺體的中間部分進行。一次性活檢針插入切口的約為30厘米的深度，並且設備被觸發到切割和收集的約2.0×0.5厘米乳腺組織的樣品。收集3個組織樣本。第一收集到的使用在確定細胞凋亡和增殖的速率，用於通過逆轉錄（RT）PCR的基因表達分析中的第二，

結果 (繁體中文) 2:

在60次哺乳時，在用ACTH或安慰劑進行挑戰後，還對8只山羊的乳腺進行活檢。8只山羊被隨機分佈，每次治療4只。每次活檢在ACTH或安慰劑後30分鐘進行。經過三角切除術、抗敗血症和麻醉術後，用手術刀在腺體的中部進行皮膚切口。將一次性活檢針插入切口，深度約為3.0釐米，並觸發設備切割並收集約2.0×0.5釐米乳腺組織的樣本。採集了三個組織樣本。第一種用於確定凋亡和增殖率，第二種用於通過逆轉錄（RT）PCR分析基因表達，第三種用於細胞培養和隨後的體外管理治療。

結果 (繁體中文) 3:

在泌乳第60天，用ACTH或安慰劑對8只山羊的乳腺進行活檢。隨機分為8只山羊，每組4只。每次活組織檢查在給予ACTH或安慰劑30分鐘後進行。三分切、消毒和麻醉後，用手術刀在腺體內側進行皮膚切口。將一次性活檢針插入切口約3.0cm深度，觸發設備切割並採集約2.0×0.5cm的乳腺組織樣本。收集了三份組織樣本。第一種方法用於檢測細胞凋亡和增殖率，第二種方法用於逆轉錄聚合酶鏈反應（RT-PCR）分析基因表達，第三種方法用於細胞培養和隨後的體外治療。