We previously reported on hair-shaft elongation from human scalp and mouse skin in Gelfoam histoculture [9, 10]. However, in the Gelfoam1 histoculture of human scalp skin, hair- shaft growth was 0.86 ± 0.18 mm over 5 days [9] and 0.7–1.10 ± 0.22 mm over 5–14 days of thehistocultured mouse skin [10]. Isolated human hair follicles in Gelfoam1 histoculture grewonly 0.49 ± 0.06 mm for 5 days [9].The present report demonstrates improved hair shaft growth of isolated hair follicles onGelfoam1. Isolated free-floating follicles were previously observed to produce elongating hair shafts but apparently were not viable for very long periods of time [12, 13]. Gelfoam1 supports both hair shaft and nerve growth [8] of isolated whisker follicles and maintained viability of the follicles for at least 63 days, much longer than free-floating follicles, which can enable longterm experimentation. Thus, the present report demonstrates an improvement of hair follicle histoculture with increased hair-shaft growth, increased period of histoculture, as well as increasing stem cell fluorescence intensity and fluorescent area for at least 63 days. The strong increase in hair-follicle stem-cell ND-GFP fluorescence indicates increased activity and the proliferation of the stem cells. It is notable that the greatest increase in stem cell fluorescence is during the period of hair-shaft elongation (Figs 2 and 3)