TOOLS provides a custom CRIPSR/Cas system in which ready-to-use system for your gene/locus will be developed upon request. Our special CRISPR/Cas System has two separated plasmids, one is sgRNA over-expressing plasmid and the other is Cas9 protein over-expressing plasmid. So, you can adjust ratio of these two plasmids to reach the best effiency during transfecion. Our sgRNA are modified to increase the binding ability with target DNA.