The molecular screening was the first step since bacterial fluoroquinolone resistance can be supported by both plasmid and chromosomal genetic elements. We attempted to determine the molecular basis of resistance phenotypes of fluoroquinolone resistant strains. After finding a mutation on chromosomal genes gyrA and gyrB, the research focused not only on the presence of plasmids, but also on fluoroquinolone resistance genes(qnr)carried by the plasmid. This study evaluated,in nalidixicacid‐resistant E.coli isolated from faeces of cattle and chicken, possible quinolone and fluoro‐ quinolone resistance through integron detection via hybridisation and subsequent polymerase chain reaction (PCR) to reveal the qnr gene. Strains were also subjected to PCR