3. Results and discussion3.1. Prepa

3. Results and discussion
3.1. Preparation of oligosaccharide by enzymatic hydrolysis
Previous experiments showed that the enzymatic activity of CZ1127 fucoidanase depends greatly on NaCl concentration, which may be a consequence of the marine environment from which the
CZ1127 bacterium strain originated. However, the high amount of NaCl hampers LC–MS detection and decreases the efficiency of oligosaccharide separation. As the result, a desalting procedure is essential after the enzymatic hydrolysis. Common methods for desalting, such as gel permeation chromatography, ion exchange chromatography and organic solvent treatment were time-consuming or ineffective for desalting sulphated oligosaccharides. The surface characteristics of nonporous graphitised carbon blacks are responsible for various types of interactions (hydrophobic, electronic and ion-exchange) with analytes, and sulphated oligosaccharides could be well retained (Hennion, 2000). After desalting by use of a nonporous graphitised carbon black column,

結果 (中文) 1:

3.结果和讨论3.1.低聚糖水解酶的制备先前的实验表明 CZ1127 藻酶的活性大大取决于 NaCl 浓度，可能是海洋环境免受其后果CZ1127 菌株起源。然而，高额的 NaCl 妨碍了液相色谱 — — 质谱检测并降低低聚糖的分离效率。作为结果，脱盐的过程是必不可少的酶水解后。脱盐，如凝胶渗透色谱法、 离子交换色谱法和有机溶剂处理了耗时或无效的脱盐硫酸寡糖的常用方法。研宄 graphitised 炭黑的表面特性是负责各种类型的交互作用 （疏水、 电子和离子交换） 分析物，与硫酸寡糖能很好保留 （亨，2000年）。通过使用无孔 graphitised 炭黑列，除盐后

結果 (中文) 3:

3。结果与讨论
3.1。通过酶水解
以前的实验制备寡糖表明海参岩藻多糖的酶的活性在很大程度上取决于NaCl浓度，这可能是由于海洋环境的
海参菌的起源。然而，盐量高，阻碍了LC–MS检测和减少寡糖分离效果fi效率。作为结果，淡化过程是必不可少的酶水解后。脱盐的常用方法，如凝胶渗透色谱，离子交换色谱法和有机溶剂处理硫酸寡糖脱盐费时或无效。无孔石墨化炭黑的表面特性是负责各种类型的相互作用（疏水性，电子和离子）与分析物和硫酸寡糖，可以很好的保留（海宁，2000）。脱盐后用无孔石墨化炭黑柱，