The mixed nutrient treatment solutions or distilled water was applied using a syringe to reach the final moisture. Then the bottles were incubated in the dark at 25 °C for 60 days anaerobic bottles using a 50-ml syringe, at 0.4 h intervals, and on day 1, 2, 3, 4, 5, 7, 9, 13, 17,20, 23, 28, 33, 40, 50, and 60 of the incubation period. Every time,approximately 35.0 ml were extracted and injected into 7.5 ml over-head bottle to be measured. In addition, samples of 20 g (wet weight)soil were collected from the plastic bottles at 3, 17, and 60 days to measure pH, Fe2+,NH4+ -N, and NO3 —N content, as well as the mi-crobial biomass carbon (MBC) and microbial biomass nitrogen (MBN).