1.precastingthe 1% agarose gel.2.dissolving 1gram of agarose into 100ml of 0.5X TBE buffer by microwaving.3.cool to 600C and pour into gel tray.4.wait 30 minutes to let the agarose to solidify.5.Mix 5ul of digested DNA, 5ul of undigested DNA with 1.2ul 6X DNA SafeViewLoading Dye and load in gel, respectively. 6.Load DNA marker by TA.7.run at 50 volt until the bromophenolblue 1/4 from the bottom.8.take a photo for record.